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dc.contributor.authorIsah, Musa
dc.contributor.authorWan Abdul Wahab, Wan-Nor-Amilah
dc.contributor.authorAbdullah, Hasmah
dc.contributor.authorJamil, Shajarahtunnur
dc.contributor.authorSul'ain, Mohd Dasuki
dc.contributor.authorUba, Abdullahi Ibrahim
dc.contributor.authorZengin, Gokhan
dc.contributor.authorLahiri, Dibyajit
dc.contributor.authorEdinur, Hisham Atan
dc.contributor.authorWan Ishak, Wan Rosli
dc.date.accessioned2024-07-03T10:10:38Z
dc.date.available2024-07-03T10:10:38Z
dc.date.issued2024en_US
dc.identifier.citationIsah, M., Wahab, W. A., Abdullah, H., Jamil, S., Sul’ain, M. D., Uba, A. I., ... & Ishak, W. R. W. (2024). Unveiling the potential antibacterial mechanism of Melaleuca cajuputi leaf extract by cell morphology studies and molecular docking analysis. Advances in Traditional Medicine, 1-12.en_US
dc.identifier.issn26624052
dc.identifier.urihttps://doi.org/10.1007/s13596-024-00763-0
dc.identifier.urihttps://hdl.handle.net/20.500.12294/4123
dc.description.abstractThe antimicrobial properties of the Melaleuca cajuputi plant have been documented. However, the underlying antimicrobial mechanisms remain relatively unexplored. Thus, this study aimed to investigate the antibacterial effects of M. cajuputi leaf extract against selected bacterial strains and unveil the potential antibacterial mechanisms of the most potent sub-fraction through time-kill assay, cell morphology studies, and molecular docking analysis. The fractions and sub-fractions were obtained from the methanolic extract of M. cajuputi leaf by bioassay-guided fractionation. The antibacterial activity was tested against Staphylococcus aureus, Streptococcus agalactiae, Klebsiella pneumoniae, and Escherichia coli using broth microdilution assay. The most potent sub-fraction, Melaleuca fraction 2d (MF2d), demonstrated remarkable antibacterial activity with MIC values ranging from 0.063 to 0.25 mg/mL and induced significant cellular damage against the tested bacteria. The chemical characterization of the most potent sub-fraction (MF2d) from methanolic extract of M. cajuputi leaf identified five (5) compounds with 2-isopropyl-10-methyl phenanthrene (83.09%) as the major component. In-silico molecular docking analysis revealed that all the docked ligands showed strong binding propensity towards target bacterial proteins, including DNA gyrase (PDB ID: 1ZI0), dihydropteroate synthase (PDB ID: 1AD1), and D-alanyl transferase (PDB ID: 6O93) with the binding energy ranging from - 6.0 to - 8.4 kcal/mol. The overall findings demonstrated the potential of the M. cajuputi plant as a valuable source of novel antibacterial agents.en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.ispartofADVANCES IN TRADITIONAL MEDICINEen_US
dc.identifier.doi10.1007/s13596-024-00763-0en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibacterial Effectsen_US
dc.subjectChemical Characterizationen_US
dc.subjectMolecular Dockingen_US
dc.subjectMelaleuca Cajuputien_US
dc.subjectScanning Electron Microscopyen_US
dc.titleUnveiling the potential antibacterial mechanism of Melaleuca cajuputi leaf extract by cell morphology studies and molecular docking analysisen_US
dc.typearticleen_US
dc.departmentFen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.authorid0000-0002-0853-108Xen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.institutionauthorUba, Abdullahi Ibrahim
dc.authorwosidP-3971-2019en_US
dc.authorscopusid57038704300en_US
dc.identifier.wosqualityQ3en_US
dc.identifier.wosWOS:001239247500001en_US
dc.identifier.scopus2-s2.0-85195182186en_US


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