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dc.contributor.authorAslan, Aşkın Sevinçen_US
dc.contributor.authorBirmingham, William R.en_US
dc.contributor.authorKaragüler, Nevin Gülen_US
dc.contributor.authorTurner, Nicholas J.en_US
dc.contributor.authorBinay, Barışen_US
dc.date.accessioned2017-07-10T12:00:29Z
dc.date.available2017-07-10T12:00:29Z
dc.date.issued2016
dc.identifier.citationAslan, A. S., Birmingham, W. R., Karagüler, N. G., Turner, N. J., Binay, B. (2016). Semi-rational design of geobacillus stearothermophilus l-lactate dehydrogenase to access various chiral ?-hydroxy acids. Appl Biochem Biotechnol. 179, 474–484.en_US
dc.identifier.issn1559-0291
dc.identifier.urihttps://hdl.handle.net/20.500.12294/845
dc.identifier.urihttp://dx.doi.org/10.1007/s12010-016-2007-x
dc.descriptionBinay, Barış (Arel Author)en_US
dc.description.abstractChiral ?-hydroxy acids (AHAs) are rapidly becoming important synthetic building blocks, in particular for the production of pharmaceuticals and other fine chemicals. Chiral compounds of a variety of functionalities are now often derived using enzymes, and L-lactate dehydrogenase from the thermophilic organism Geobacillus stearothermophilus (bsLDH) has the potential to be employed for the industrial synthesis of chiral ?-hydroxy acids. Despite the thorough characterization of this enzyme, generation of variants with high activity on nonnatural substrates has remained difficult and therefore limits the use of bsLDH in industry. Here, we present the engineering of bsLDH using semi-rational design as a method of focusing screening in a small and smart library for novel biocatalysts. In this study, six mutant libraries were designed in an effort to expand the substrate range of bsLDH. The eight variants identified as having enhanced activity toward the selected ?-keto acids belonged to the same library, which targeted two positions simultaneously. These new variants now may be useful biocatalysts for chiral synthesis of ?-hydroxy acids.en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChiral ?-Hydroxy Acids (AHAs)en_US
dc.subjectGeobacillus Stearothermophilus L-Lactate Dehydrogenase (bsLDH)en_US
dc.subjectProtein Engineeringen_US
dc.subjectModelingen_US
dc.titleSemi-rational design of geobacillus stearothermophilus l-lactate dehydrogenase to access various chiral ?-hydroxy acidsen_US
dc.typearticleen_US
dc.relation.journalAppl Biochem Biotechnolen_US
dc.contributor.departmentİstanbul Arel Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.contributor.authorIDTR16264en_US
dc.identifier.volume179en_US
dc.identifier.startpage474en_US
dc.identifier.endpage484en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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